Escario5 1. Recibido X Corregido Vi Aceptado Vii Abstract: Steroidal saponins from the plant Agave brittoniana with activity against the parasite Trichomona vaginalis. The genus Agave Agavaceae , includes more than species; around 16 of them show an homoge- neous distribution throughout Cuba. Agave brittoniana ssp. The parasite Trichomonas vaginalis causes the disease known as trichomoniasis, that infects the genital tract.
To test in vitro the plant against Trichomona vaginalis, the dried and powdered leaves were extracted three times with ethanol-water 7 : 3 by maceration at room temperature. The solvent was removed under reduced pressure and the extract was suspended in distilled water, defatted with n-hexane, and extracted with water-saturated n-butanol. After solvent removal, a portion of the n-butanol extract was hydrolyzed. After extraction with ethyl acetate the hydrolysis products were compared with authentic sapogenins samples using thin layer chroma- tography TLC.
Most of the sapogenins yuccagenin and diosgenin were isolated and their structures were confirmed. The n-butanol extract was subjected to a separation process through column chromatography to obtain five fractions. After multiple separation processes by reversed phase high performance liquid chromatography HPLC , the most active one produced one refined fraction that contained two saponins with the same aglycone diosgenin and one yuccagenin based saponin.
Best results of the activity were obtained with the yuccagenin derived glycoside. Epub December Key words: Anti-parasitic, Agave brittoniana, Trichomona vaginalis, steroidal saponins. ISSN Vol. La sola prueba de espuma positiva no es concluyente para determinar la presencia de saponinas. Si la muestra contiene taninos, estos pueden eliminarse pues se absorben en MgO. Budzikiewicz y col.
Un ejemplo de su utilidad se observa para el caso del espectro de 14 Abdel-Gawad, M. Los protones del C resuenan en 3. Los protones del metilo resuenan como un singlete en 0. La figura 7 muestra algunos ejemplos de medicamentos esteroides producidos a partir de esteroides naturales. La 28 Hu, K. Park Press, State Coll. El fique, el cual es usado por los campesinos para elaborar canastas y productos artesanales, se obtiene de las hojas de Agave sp.
Daniel J. Guadalupe, O O Etisterona Figura O Compuesto S Aspergillus sp. Gnoatto, S. Higgins, J. Kohara, A. Liagre, B. Lu, M. Man, S. Martinez, A. Merlani, M. Musende, A. Oleszek, W. Osorio, E. Rattanathongkom, A. Planta Med. Saleem, M. San Martin, R. Sharapin, N. Principios de Analisis Intrumental. Quinta Edicion, Mc Graw Hill. Sun, S.
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